Effects of dietary calcium, phosphorus and microbial phytase on intestinal morphologyin laying hens

Different challenges are being applied in the poultry industry order to protect animal health and increase immunity production. The supplementation of microbial phytase is essential terms both reducing inorganic rate contributing absorption other minerals. In this study, a newly isolated was added at different concentrations diet together with calcium (Ca$^{2+}$) available phosphorus (AP), effects on intestinal capacity Ca$^{2+}$ binding were investigated via morphological measurements immunohistochemical examination duodenum ileum. For purpose, 90 Lohmann LSL-White laying hens divided into three main groups: 1. Standard AP (Ca+AP), 2. low (Ca+low AP), 3. Low Ca2+ (low Ca+low AP). These groups further subgroups each (without [Phy-], commercial [CP] [MP]). At end experiment, animals euthanized, ileum samples fixed processed for histological examination. Villus height, crypt depth, total mucosa thickness, villus width measured height: depth ratio area calculated. Caldesmon expression also immunohistochemically. results indicated that increased (p ≤ 0.05) birds fed Ca+AP+MP. Stronger caldesmon observed MP treated groups. We concluded produced from Bacillus megaterium EBD 9-1 bacterium increases utilization and, thus, can have beneficial role when these macrominerals used insufficiently. Ca$^{2+}$, AP, may positive morphology optimum amounts.